Association of Polymorphism at 3׳-UTR of Urokinase Gene with Risk of Calcium Kidney Stones

Assari, Neda; Angaji, SeiedAbdolmajid; Morovvati, Saeed

Association of Polymorphism at 3׳-UTR of Urokinase Gene with Risk of Calcium Kidney Stones

Document Type : Article

Authors

¹ Department Of cell and Molecular biology, Kharazmi University

² Department of Molecular and Biology, Faculty of Medical Sciences, Baghiyatallah University of Medical Sciences, Tehran, Iran

Abstract

Urokinase might play a role in the formation of kidney stones. This study was done to determine the association between +4065 T/C polymorphism at the 3′-untranslated region of urokinase gene and calcium kidney stones. This Case-Control study was carried out on 70 cases with a history of calcium kidney stones and 70 controls from the Baqiyatallah hospital of Tehran in 2013. The study of polymorphism was performed by Allele-specific PCR method. Mutant T allele frequency was %41 in cases and %18 in controls. Hardy-Weinberg equilibrium (HWE) was tested using chi-square test. The chi-square showed that the control population is in HWE, and as expected, the patient group does not follow HWE. The odds ratio for the risk of the T allele was 1.7 (%95 confidence interval 0.2988-1.158). A significant association was found between the urokinase gene T/C polymorphism and the formation of calcium kidney stones. Also, effect modification was examined for both sexes and different age groups. According to the results, T polymorphism in the 3’-UTR of urokinase gene may increase with age.

Graphical Abstract