Altered Expression of Epidermal Growth Factor Receptor (EGFR) in Glioma

Document Type : Article

Author

1. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran.

Abstract

      EGFR is a key molecule in cancer cells. EGFR signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. We extracted proteins from astrocytoma (III and IV) oligodendroglioma(IV) tumors and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometry method. In this study, we separated proteins by the two-dimensional gel (2DG) electrophoresis method, and the spots were analyzed and compared using statistical data and Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-TOF MS). EGFR position showed in the diagram cluster for oligodendroglioma tumors (t-test and one-way ANOVA; P < 0.05). EGFR proteins were definitely with isoelectric pH, molecular weight and protein databank detected which has an up-regulation. Proteomics analysis revealed an unexpected alteration in the expression of certain protein networks in EGFR gliomas. The EGFR dependent signaling pathways are most frequently reported in high grade oligodendroglioma and affect all histological closes. Thus EGFR could be a candidate biomarker in glioma tumors.

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Altered Expression of Epidermal Growth Factor Receptor (EGFR) in Glioma

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References

 
 
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